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Washed beads were resuspended in 100 L of 50 mM HEPES buffer (pH 7.4) and sequentially reduced with TCEP (200 M) and re-alkylated either with IAM (400 M) Procedure A or with IAM-TPP (400 M) Procedure B for 30 min at RT in the dark

TRPV1 hyperfunction contributes to renal inflammation in oxalate nephropathy

The free-energy landscape built with the first two eigenvectors of the PCA analysis showed a less rugged surface and a smaller number of energy minima in the holo-form, in contrast with the behavior of the apo-form (Fig 2)

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347,348 Aging-related NAD + decline causes mitochondrial dysfunction Combining the evidence that mitochondrial dysfunction is a hallmark of aging and NAD + plays a crucial role in the maintenance of mitochondrial function